Department of Cell Biology
333 Cedar Street
PO Box 208002
New Haven, CT 06520-8002
Tel: 203.785.4311
Fax: 203.785.7446

Electron Microscopy

Fees (2007-2008)

1. Complete service

	Yale	Other Educational Institution	Industry
Regular EM / sample	$ 200	$ 230	$ 400
Immuno-EM / sample	$ 450	$ 518	$ 900

Complete service includes embedding, ultrathin sectioning/cryosectioning, staining/immunogold labeling, observation at the EM, and the production of 10 representative digital images, all carried out by the staff of the facility.

2. Services

	Yale	Other Educational Institution	Industry
Staff Time / hour	$ 55	$ 63	$ 110
Embedding (1-5) / sample Embedding (>6) / sample	$ 60 $ 50	$ 69 $ 58	$ 120 $ 100
Plastic sectioning / sample	$ 55	$ 63	$ 110
Cryo-sectioning / sample	$ 120	$ 138	$ 240
Single Immunolabeling / run	$ 175	$ 201	$ 350
Double Immunolabeling / run	$ 285	$ 328	$ 570
Cryoprotection and Rapid Freezing / sample	$ 120	$ 138	$ 240
Freeze-Fracture / sample	$ 300	$ 345	$ 600
Negative Staining / sample	$55	$ 63	$ 110

Plastic sectioning includes staining of one grid with lead citrate and uranyl acetate. The charge for cryo-sectioning is for a maximum of 8 grids, additional grids being charged per time. The charge for immunolabeling is for a maximum of 20 grids and/or 5 different antibodies per run.

3. Instrument rental

	Yale	Other Educational Institution	Industry
EM time (unassisted) / hour	$ 45	$ 52	$ 90
EM time (assisted) / hour	$ 100	$ 115	$ 200
Ultramicrotome / hour	$ 18	$ 21	$ 36
Cryo-ultramicrotome / hour	$ 30	$ 35	$ 60
Cryostat / hour	$ 30	$ 35	$ 60
Evaporator / run	$ 20	$ 23	$ 40
Diamond Knife / hour	$ 20	$ 23	$ 40

4. Laboratory supplies

	Yale	Other Educational Institution	Industry
Coated Grids (ea)	$ 1.50	$ 1.75	$ 3
Embedding Supplies	$ 30	$ 35	$ 60
EM Negatives	$ 2	$ 2.30	$ 4
Pictrography 3000 Digital Prints (ea)	$ 10	$ 12	$ 20

Yale School of Medicine

Center for Cellular and Molecular Imaging

Electron Microscopy

Fees (2007-2008)