Marc Pypaert, Ph.D.

Research Scientist, Cell Biology Director of Electron Microscopy Core Facility
Phone: (203) 785-3681 Cell: (203) 927 4265 Lab: (203) 785-5390 Fax: (203) 785-7446 e-mail: marc.pypaert@yale.edu		Department of Cell Biology Yale Universtiy School of Medicine 333 Cedar Street PO Box 208002 New Haven, CT 06520-8002 <Courier Address> 333 Cedar Street, SHM IE-16 New Haven, CT 06510-3206

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As part of the Center for Cell and Molecular Imaging (CCMI), the Electron Microscopy Core Facility provides expert assistance in a vast array of electron microscopy techniques to investigators within the School of Medicine and the Yale research community.  One of the main specialties of the laboratory is immuno-gold immunocytochemistry on ultra-thin cryosections.  Combined with stereology, this technique allows the quantitative characterization of the distribution of proteins in cells and tissues at a level of resolution unattainable by light microscopy. Other techniques available in our laboratory include regular morphology of plastic-embedded samples, stereology, scanning electron microscopy, freeze-fracture and rotary-shadowing, three-dimensional reconstructions from serial sections, low-temperature embedding and negative staining.

Selected Publications

Kim, S. V., Mehal, W. Z., Dong, X., Heinrich, V., Pypaert, M., Mellman, I., Dembo, M., Mooseker, M. S., Wu, D., and Flavell, R. A. (2006) Modulation of cell adhesion and motility in the immune system by Myo1f. Science 314:136-139.

Shin, J. S., Ebersold, M., Pypaert, M., Delamare, L., Hartley, A., and Mellman, I. (2006) Surface expression of MHC class II in dendritic cells is controlled by regulated ubiquitination. Nature 444:115-118.

Schmid, D., Pypaert, M., and Munz, C. (2007) Antigen-loading compartments for major histocompatibility complex class II molecules continuously receive input from autophagosomes. Immunity 26:79-92.

Unternaehrer, J. J., Chow, A., Pypaert, M., Inaba, K., and Mellman, I. (2007) The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc. Natl. Acad. Sci. U.S.A. 104:234-239.

Reznick, R. M., Zong, H., Li, J., Morino, K., Moore, I. K., Yu, H. J., Liu, Z. X., Dong, J., Mustard, K. J., Hawley, S. A., Befroy, D., Pypaert, M., Hardie, D. G., Young, L. H., and Shulman, G. I. (2007) Aging-Associated Reductions in AMP-Activated Protein Kinase Activity and Mitochondrial Biogenesis. Cell Metabolism 5:151-156.

Shang, Y., Claridge-Chang, A., Sjulson, L., Pypaert, M., and Miesenbock, G. (2007) Excitatory local circuits and their implications for olfactory processing in the fly antennal lobe. Cell 128:601-612.

Sherer, N. M., Lehmann, M. J., Jimenez-Soto, L. F., Horensavitz, C., Pypaert, M., and Mothes, W. (2007) Retroviruses can establish filopodial bridges for efficient cell-to-cell transmission. Nature Cell Biol. 9:310-315.

Lab Members:

Marc Pypaert, Ph.D.
Research Scientist
Director of EM Facility
Office: SHM IE-16
Tel (203) 785 3681
Mobile (203) 927 4265
Fax (203) 785 7446
marc.pypaert@yale.edu

Christoph Rahner, M.D.
Associate Research Scientist
Office: SHM IE-14
Tel (203) 785 4322
Fax (203) 785 7446
christoph.rahner@yale.edu

Kimberly Zichichi
Research Assistant
Office: SHM IE-26
Tel (203) 785 5371
Fax (203) 785 7446
kimberly.zichichi@yale.edu

Christina Horensavitz
Research Assistant
Office: SHM IE-14
Tel (203) 785 7943
Fax (203) 785 7446
christina.horensavitz@yale.edu

Morven Graham
Research Assistant
Office: SHM IE-14
Tel (203) 785 7943
Fax (203) 785 7446
morven.graham@yale.edu

Main Laboratory
SHM IE-26
Tel (203) 785 5390