Joerg Bewersdorf PhD
Associate Professor of Cell Biology and of Biomedical Engineering
Super-resolution fluorescence microscopy
We are currently working on multiple projects to further improve fluorescence imaging technology and applying these cutting-edge techniques to current biological questions.
Visualizing 3D structure and dynamics at the molecular
scale is a current and critical need in biomedical research. Many sub-cellular
features, for example the morphology of many organelles or the 3D organization
of chromatin, cannot be resolved by standard light microscopy.
Improving the resolution of light microscopy has therefore been an urgent need of biological research for many decades. Today, several methods achieve sub-100 nm resolution by taking advantage of reversible or irreversible photo-physical switching properties of fluorescent markers.
Our research group in the Department of Cell Biology at Yale University School of Medicine is developing new fluorescence microscopy techniques with spatial and/or temporal resolutions exceeding far beyond current technology and also applying them to a diverse set of biological questions.
Extensive Research Description
Our laboratory works on both Stimulated Emission Depletion (STED) microscopy and Fluorescence Photoactivation Localization Microscopy (FPALM/PALM/etc.) techniques. Since October 2009, we have one of the first Leica TCS STED microscopes in the United States in our laboratory.
Additionally, we use 3D 'Biplane FPALM' instruments in current morphological studies of vesicle trafficking and 3D chromatin structure. Moreover, we are in the process of setting up next generation instruments with higher spatial or temporal resolution for tomorrow's cell biological research.